![]() Second, the gene-of-interest is then packaged into an infectious virion via the expressed viral particles. First, a cell is transfected with numerous plasmids encoding the gene-of-interest along with various viral particles. Transduction, on the other hand, is a multi-step process. 7 For transformation (into bacteria) or transfection (into mammalian cells), the cell will express the recombinant protein. Liposomes are also employed to deliver DNA to cells (Figure 2). 5 In either case, the plasmids encoding the gene-of-interest passively enter the cell through its membrane, which has been temporarily compromised (i.e., “holy”) via calcium phosphate or electroporation. The viral vector flanks the gene-of-interest by viral sequences, which eventually result in the gene being inserted into the host chromosome permanently.Īfter cloning, genes encoding for the gene-of-interest are introduced into the host system, either transiently by a cDNA plasmid (i.e., transformation, transfection) or permanently by viral infection (i.e., transduction). The antibiotic resistant gene enables the selection of cells carrying the plasmid in antibiotic-based media. It also often has a sequence at the N- or C-terminus encoding a fusion tag for downstream protein purification or identification. 5,6 An expression vector is a cDNA plasmid that includes a promoter sequence and an antibiotic resistant gene. The synthesized gene is then cloned into an expression or viral vector. The efficiency of protein translation is improved by optimizing the nucleic acid sequences that reflect the codon bias (due to the varying tRNA pools) of the chosen expression system. For example, four codons (CTT, CTC, CTA, CTG) will be translated to the amino acid residue, leucine. ![]() The nucleic acid sequence is important to consider since one amino acid is encoded by different 3-nucleic acid sequences called codons. The first step of making a recombinant protein is synthesizing the gene-of-interest. ![]()
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![]() ![]() Coach Howland has 3 big wins already on this year’s résumé, come conference play, he’ll be ready to add many more to that list. Wright State’s roster is very guard heavy, and they’ll need every single one of them to try and slow down the red-hot guards that are playing in Maroon & White. This will be a great test for Ado to become a more versatile defender. Both players are 6’11”, but Ernsthausen has the ability to shoot the ball from 3. The biggest matchup I would pay attention to is Mississippi State’s Abdul Ado against Parker Ernsthausen. Buy Wright State Raiders Embroidery College Logo and get 4 sizes and 8 formats in one. Wampler is currently scoring 14.7 points per game. Wright State’s leading scorer is Junior Forward Billy Wampler. I believe that Coach Ben Howland has the ability and the experience on the team to prevent that from happening. I don’t see this as a test to see if Wright State can beat Mississippi State, but there is a test to see if the Bulldogs can focus on this game and not look ahead. Wright State got out of that slump and is coming in after two straight wins against Northwest Ohio and Morehead State. Currently over 10,000 on display for your viewing pleasure. A virtual museum of sports logos, uniforms and historical items. 5 of those losses came in a 6 game stretch. Wright State Raiders Logo on Chris Creamer's Sports Logos Page - SportsLogos.Net. Wright State brings in a team with losses to Murray State, Penn State, SMU, Indiana State, Miami (Ohio), and Kent State. Teams like Clemson and Cincinnati have fallen victim to the Bulldogs’ excellent shooting in the past few weeks. Mississippi State is one of the hottest shooting teams in the country and no team has been able to stop them. Tonight, Mississippi State (10-1) hosts Wright State (6-6) at The Hump as the second-to-last non-conference game. ![]() ![]() ![]() Regarding Google Translate, I have these two im my bookmarks: Textify opens the Dev edition instead of the Release edition. I have 2 editions of Firefox installed: Release (=default) and Dev. Use the “More settings” button in Textify for details. In addition to that, it’s now simpler to configure a button to launch a custom command, be it a URL or a program on your computer. Firefox doesn’t provide such an option, but it provides the -width, -height, -new-window command line options which allow to create a small popup window, albeit with the URL bar and the rest of the UI. Chrome provides an option to create a new minimalistic window without the URL bar by using the -app command line switch. Currently, Chrome and Firefox are supported. Textify v1.8 improves the situation by opening the website in a new popup window while using the default browser. That’s too distracting, at least for me, especially when I’m already using the browser and need a quick translation or search. Using the default browser solves the issues above, but the website opens in a new tab for most browsers. Finally, you had to login to websites separately, since IE is probably not the browser you use daily. Also, several websites (again, Google Translate is an example) showed an offer to upgrade to a better browser right in the popup. The IE control popup was often slow to load, especially for the Google Translate website. Using the feature for a while, I discovered that each option has its own drawbacks. In the original implementation, you could configure a button to open a link in one of the following two ways: in a popup window which hosts an IE control, or in the default browser. News from the Wire TAG launches Textify Mobile text marketing and fundraising unit Friday, Ap11:28:00 (EDT)īrentwood, Tenn., Ap- The A Group (TAG), a marketing and technology agency in Nashville, Tennessee, announces the launch of a sister company, Textify Mobile, a software-as-a-service startup designed to provide businesses and organizations with an easy-to-use text marketing and fundraising solution.About two years ago I introduced the so-called “web buttons” in Textify in the Textify v1.6 meets the web post. Textify was created through a unique entrepreneurial model that gave TAG employees the opportunity to be a part of the launch team in exchange for profit sharing. Just weeks after launch, Textify is already witnessing great success, with nonprofit users raising thousands of dollars and acquiring hundreds of new donors using the texting platform. “There are more cell phones than toothbrushes in the world,” said Maurilio Amorim, Founder and CEO of The A Group and Textify Mobile. It allows users to manage text-based fundraising event. Texting is critical to reaching an audience wherever they are.” “Our phones are almost always within an arm’s reach, and most texts are read within minutes of being sent. Textify offers a text-based fundraising tool for churches and organizations to raise money. Textify is an easy-to-use and affordable text marketing and fundraising platform that gives businesses, artists, churches, and nonprofits the ability to communicate with supporters, recruit volunteers, send out special offers, accept donations, and sell products, all from a phone. “Rather than try to fit our them into existing solutions we weren’t happy with, we decided to dream up our own.” “We saw an incredible need in the market as businesses and organizations were realizing how critical it was to have a text strategy, but weren’t finding solutions to meet their needs,” said Amorim. The idea for the product was born from The A Group’s decade of experience working with clients – mainly churches, ministries, and nonprofits – who were seeking mobile marketing solutions but unable to find one service that met all of their needs. With a team of marketers and developers in house, TAG decided: why not build their own. “We wanted to challenge our team to build something innovative while creating an opportunity for them to become entrepreneurs and reap the rewards of a starting company,” said Amorim. TAG employees who opted to be a part of the startup worked after hours as contractors to develop, brand, and market the software in exchange for payouts from the profits and future profit sharing in Textify. ![]() ![]() ![]() What should we do? After reading more about the chemicals involved in vaping and their effects, I believed more than ever that treatment for ocular surface disease (OSD) includes educating patients on natural and self-induced environmental challenges. ![]() Related: Diving into dry eye can help physicians solve patients' woes Schirmer’s tests were inconsistent in the vaping group, and may be attributed to compensatory lacrimal gland output phases of dry eye disease, which can burn out over time. One recent paper looked at ocular surface findings in vapers versus non-vapers and found that vapers had staining and compromised tear breakup time. Researchers are now studying OSD and vaping. The reputation of the brand does not confer pulmonary or other organ system safety to our patients. Without regulation, it is impossible to know if or when other potentially dangerous substances will be added to vaping fluid in the future. ![]() Related: Nutrition a priority for dry eye patients The sticky ester was used as a filler, but it is not safe for inhalation. 7Ī retrospective analysis of the vaping fluid composition showed that 100% of the cartridges used by the affected patients contained vitamin E, which had not been present in the same cartridge brand in the past. The rash of cases where vapers experienced severe lung toxicity requiring lung transplants occurred after a change in manufacturing resulted in the addition of vitamin E to some vaping cartridges. Related: New for 2020 and beyond: Dry eye disease agents in pipeline 6 Other potential components have additional health implications as well, including nitrosamines, benzaldehyde, benzyl alcohol, propylene glycol, and ethylene glycol. Some vaping chemicals may also activate the immune mechanism of chronic DED. 4Ĭarriers and flavor additives can also cause lung toxicity and directly activate the transit receptor potential (TRP) of the ocular surface immune system and corneal nerves. In addition to nicotine, vaping fluid components include acrolein (2-propenal), an aldehyde that severely limits regulatory t-cells - a mechanism that can thereby activate and contribute to chronic dry eye disease (DED). Vaping fluid is packed with other chemicals. Related: Treating dry eye with recombinant human nerve growth factor Photo credit: Laura M Periman MDĮffects of nicotine on the eye include pupil constriction 2 and reduced retinal activity visible on electroretinogram (ERG). Pictured: A 36-year old ocular surface disease (OSD) meibomian gland dysfunction (MGD) patient who frequently uses vaped THC. 1 This includes seizures, anxiety, depression, cognitive compromise, and inattention. Unlike cigarettes, which contain the amount of nicotine that naturally occurs in tobacco, vaping fluid contains extracted nicotine in levels that are uncontrolled and often unknown.ĭoctors are seeing nicotine-induced illness (“nic-sick”), particularly in teens. Periman: Ophthalmology can 'adopt' technologies from other specialties ICD-10-CM H57.04 is grouped within Diagnostic Related Group(s) (MS-DRG v41.Previously by Dr. This condition may occur due to injury of the pupillary fibers in the oculomotor nerve, in acute angle-closure glaucoma, and in adie syndrome. Dilation of pupils to greater than 6 mm combined with failure of the pupils to constrict when stimulated with light.symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified ( R00- R94).injury, poisoning and certain other consequences of external causes ( S00-T88).injury (trauma) of eye and orbit ( S05.-).endocrine, nutritional and metabolic diseases ( E00- E88).congenital malformations, deformations, and chromosomal abnormalities ( Q00-Q99).complications of pregnancy, childbirth and the puerperium ( O00-O9A).certain infectious and parasitic diseases ( A00-B99).certain conditions originating in the perinatal period ( P04- P96). ![]() ![]() It is nice to just sit back and watching the splashing waves around the boat while it is surfing in the water. ![]() And you can get a over-distance alert and low battery alarm from controller, delivering you a worry-free trip.Īmazing Auto-pilot Mode: With just one click in the remote controller, this rc boat can give you “O” shape demonstration. Kids can have fun together.The control range of this racing boat is up to 394FT. Smart Remote Control and Wide Control Range: Smart 2.4 GHZ gives you and kids non-jamming non-interference remote control. The flashing blue LED light is much much more brighter and cooler than the ones in the market, giving you the day & night fun. Self-righting Hull Design & Bright LED Light For Kids & Adults: This jet boat features easy control, capsize recovery mode can turn the boat upright if it flipped. Running the boat with your kids swimming in the pool is perfectly safe as there is no exposed propellers and won’t accidentally scratch anyone. This racing boat won’t easily catch grass, leaf or other stuff in the water as the propeller is nicely hidden inside the body. Safe Jet Pump Propulsion System: The newest turbojet design with hidden propellers and motors, making this rc boat suitable for much more different waters, not just limited to lake or pools. 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Exquisite workmanship, mini and lightweight. 【High Simulation Submarine】Waterproof ABS plastic parts are durable and smooth. 【Specifications】Submarine Battery: 3.6V 120mAh Ni-MH Battery, Charging Time: 25 mins, Working Time: 5-10 mins, Control Distance: 5m / 16.4ft, Submarine size: 4.9 x 1.6 x 2.2 inch The Remote Controller requires 2 AAA batteries (Not Included) 【Easy to Charge】The submarine integrates a built-in rechargeable battery, and it can be charged by USB charging cable. When put it into water, the submarine will be activated and the head lights will turns on automatically. 【Water Activated】There is no switch on the submarine. There are also two navigation lights at the head of the submarine, which will light up when sailing in the water. 【Multifunction】It is a 6 channel RC toy which can go forward/backward, turn left/right, dive, float upward. 【Remote Control Submarine】This RC submarine can drive on the water surface or dive into water unders instructions from the controller, just like a real nuclear submarine sailing in ocean. ![]() ![]() Export: Batch-process multiple photos at once and export them to various formats with flexible settings. ![]() Develop: Make global and local adjustments to refine your images and bring out the best in your photography.Advanced Search: Use facial recognition and metadata filters to quickly find photos.Integrates with Facebook and Instagram.In conclusion, most professional and amateur photographs alike can get a lot done with Adobe Photoshop Lightroom Classic and we'd really encourage anybody looking into photography to have a look at this free trial application before purchasing any other package.įeatures of Adobe Photoshop Lightroom Classic And in fact, presets might be the most powerful feature of Adobe Photoshop Lightroom Classic. Worth noting is that graduated and radial filters can also be included in presets. Virtually all edits using the different levels can be applied with graduated or radial filters. Presets are essentially a collection of pre-made adjustments that can be applied one-by-one or en masse to a set of photographs. Quick editing with existing or downloadable presetsįor those who have a certain set of favorite adjustments, Adobe Photoshop Lightroom Classic can create what are called presets. Labels as well as ratings can also be added to images. Sharpening pictures is a breeze with several different options available but the included presets are a good place to start.Īn image's histogram, cropping, red eye removal or spot tool are features available, along with radial and graduated filters. Presence adjustments are clarity, vibrance and saturation for creation of different types of effects including HDR photography. Editing workflow can include adjustment of levels: exposure, contrast, highlights, shadows, whites, blacks and temperature plus tint. When it comes to editing digital photographs, Lightroom Classic is completely packed with features. Great way to edit and optimize raw digital photographs Lens profiles are one of the most important features of Adobe Photoshop Lightroom Classic that provide distortion corrects and removal of chromatic aberration. Other smaller brands are supported too including Apple's iPhone, Samsung's Galaxy devices and more. ![]() The program is optimized for batch processing in mind and contains all of the support needed to process raw image files from virtually all makes of cameras, including Sony, Canon and Nikon. Excellent image enhancement and processing software that has image editing features, photo process workflow, presets and profiles for major makes and models of camera including cell phones.Īdobe Photoshop Lightroom Classic is an excellent, if not the best RAW image processor and workflow application for photographers. ![]() ![]() ![]() ![]() I just said, 'Yep, it's because those people back at the stadium were praying for me.'.ĭo I believe people ought to wear masks ? Yep, I believe people ought to wear masks. That's progress.I got to the hospital, and for some reason, I lost the numbing feeling in my back, i had all feeling and everything I could possibly get back. While Newsbusters' Brent Baker was appalled that ABC News had "elevated" the "left-wing hit," he didn't quite go so far as to explicitly say the comment wasn't racist. He’s a Harvard man." (Thomas received his law degree, by contrast, from plebian institution Yale.)īut maybe that practice is starting to sink in. He does think he’s smarter and better than everybody else. In 2010, Harvard professor Charles Ogletree said Sarah Palin's habit of deriding Obama as a "professor" was code for "uppity." Limbaugh responded by saying the term was racist when applied to Clarence Thomas, but true when applied to Obama: "Obama is uppity, but not as a black. This is especially curious because Westmorland is from Georgia. Lynn Westmorland claimed he didn't know "uppity" had racial connotations when he used the term to describe then-Sen. Even so, they've had a lot of practice with uppity in recent years. Maybe these media guys don't know how to Google. The most liked and most disliked definition at Urban Dictionary notes that "uppity" is often followed by the n-word. ![]() But a little more digging could help these guys out. Various forms of the question "Is uppity racist?" is a very popular on Yahoo Answers. In fairness, a lot of people don't know for sure whether "uppity" is racist. We're living in a country where you can't say that's a little uppity?"īeck seemed unaware "uppity" was a term racist southerners used for black people who didn't know their place. He continued, "They understand it is a little bit of uppity-ism." Glenn Beck defended the comment, saying on Imus' radio show, "Uppity? You don't think she's a little snotty? Really? Really? Miss Arugula? Come on!" ( Arugula is a type of lettuce that is offensive to some conservatives.) "I'm not going to apologize for saying the woman who says 'I'd like a good steak and arugula once in a while'. are mature, tolerant people who fully understand when they’re being insulted and condescended to," Limbaugh said, then listed Obama's transgressions such as taking expensive vacations and saying exercise is good. It's hard to explain how they've managed to avoid finding out about "uppity" secret past. Glenn Beck, too, is defending Limbaugh's analysis, saying it's just a synonym for snobby. This week it's Rush Limbaugh, who said Michelle Obama was booed at a weekend Nascar race because she showed "uppity-ism," as well as the conservative site Newsbusters, which is just shocked that anyone might call that comment racially problematic. President Obama has been a well-known public figure for several years and his conservative critics, in particular, keep making the "uppity" mistake. Ī lot of people have no idea that the word "uppity," when applied to black people, has racist connotations, but it's getting harder and harder to understand how public figures, in particular, are able to maintain their ignorance of the term's history. This article is from the archive of our partner. ![]() ![]() ![]() Our live chat is not only for picking up orders, have a question before placing an order? Feel free to ask us anything. No Matter where you live, we are online! Our 24/7/35 Live Chat is ready whenever you need us. Looking to buy more? 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